PO-04-063 EFFECT OF PULSED FIELD ABLATION ON THE BRONCHUS: PRECLINICAL INSIGHTS

The relative tissue selectivity of pulsed field ablation (PFA) allows for the safe delivery lesions even when in close proximity to collateral tissues such as esophagus and phrenic nerve. Frequent coughing spells during PFA left atrium can be frequently seen clinical procedures; there is little insight into mechanism effects on bronchi. To assess effect bronchus swine. Microsecond pulses were delivered a single swine under general anesthesia with fluoroscopic guidance. A standard catheter (Blazer II, Boston Scientific, 7Fr 4mm tip) commercially available microsecond pulse generator (ECM 830, BTX) used. Bipolar, biphasic mode (750kV) was applied 5 repetitions ∼1 second-long applications within bronchus. Tetrazolium chloride staining performed humanely sacrificed after 2 days. Histological assessment formalin fixation Hematoxylin Eosin staining. total 7 created both right Only identified at necropsy (Figure). oval shape pink-white center surrounded by zone hyperemia. Histology demonstrated disappearance cilia mucosal cells site compared adjacent normal tissue. layer respiratory epithelium denuded. Structures beneath epithelium, including lamina propria, smooth muscles, mucosa glands, cartilage spared without evidence any hemorrhage or inflammation. This exploratory sampling bronchial susceptibility suggests minimal damage epithelium. Detailed post-PFA bronchoscopy studies would necessary required if are atrial procedures.